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CRISPR Gene Editing: Methods and Protocols

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CRISPR Gene Editing: Methods and Protocols

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successfulÂ Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Â

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.Â

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
Country USA
Brand Humana
Manufacturer Humana
Binding Hardcover
ItemPartNumber 54647760
ReleaseDate 2019-03-26
UnitCount 1
EANs 9781493991693

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